Recovery from hemorrhage-induced anemia in WT and Erfe^−/− mice. Hemoglobin (A) and hematocrit (B) levels in 7- to 9-week-old WT (blue) and Erfe^−/− (red) male mice 0, 1, 2, 3, 4, 5, and 6 days after phlebotomy (500 μL). (C) Time course of serum ERFE concentration in phlebotomized WT mice. mRNA expression of Hamp (D), Id1 (E), and Smad7 (F) in the liver of phlebotomized WT and Erfe^−/− mice. Data shown are mean ± standard error of the mean (SEM) and were compared for each time point with values for control mice at t = 0 (n = 5-8) for each genotype by 2-way (A,B,D,E,F) or 1-way (C) analysis of variance (ANOVA) and corrected for multiple comparisons by the Holm-Šidák method. ∗∗∗∗P < .0001; ∗∗∗P < .001; ∗∗P < .01; ∗P < .05.