Overview of technologies for evaluating the cellular composition of tissues. (A) Spatial technologies organized by resolution (x-axis) and volume of tissue that can be profiled (y-axis). Number of analytes (∗) and resolutions are provided as an estimate only. These numbers are based on the literature44,82-87 and discussions with method developers. Numbers are subject to change as technology improves. Subcellular, <1 μm; single cell, 1-9 μm; multicell, 10-50 μm; and Ecosystem, 500 μm. (B) Simplified depiction of spatial approaches. Antibody-based detection∗ for spatial proteomics denotes that only optical microscopy approaches are represented here not IMC or MIBI-TOF. (C) Broad overview of technologies that can be applied to cell suspensions prepared from dissociated tissues. CITE-Seq, cellular indexing of transcriptomes and epitopes by sequencing; FISH, fluorescence in situ hybridization; H&E, hematoxylin and eosin; IHC, immunohistochemistry.
