Spatial organization and cellular composition of non-FL and FL LNs from human patients. Cell DIVE⁴¹ imaging platform and IBEX⁴² dye inactivation protocol were combined for high parameter imaging (Cell DIVE-IBEX). Changes in the organization and cellular composition of FL LNs as compared with non-FL (normal) LNs. B-cell follicle outlined by dotted line. Arrows denote medullary or subcapsular sinus enriched with DC-SIGN⁺ myeloid cells. Scale bar, 150 μm (large images) and 50 μm (small images). LZ and DZ of GC. Example Tfh cells marked by asterisks. IBEX, iterative bleaching extends multiplexity.