Figure 1. Inhibition of aPL... | American Society of Hematology

Figure 1.

$Inhibition of aPL prothrombotic and proinflammatory response by NAPc2. (A) Thrombus formation in mice treated with immunoglobulin (10 μg) fractions either alone or together with NAPc2; platelets are shown in red, and leukocytes are shown in green. Quantification of thrombus size in the vena cava inferior 3 hours after injection of immunoglobulin isolated from healthy controls (n = 4) or patients with COVID-19 (n = 6) and flow restriction. Mean ± standard deviation (SD), ∗∗∗∗P < .0001, t test following Shapiro-Wilk test for normal distribution. (B) MM1 cells were cultured in human serum or plasma and then stimulated for 1 hour with HL5B or HL7G (400 ng/mL each), rJGG9 or IgG (1 μg/mL each), LPS (10 ng/mL) or IgG from patients with COVID-19 (10 μg/mL). (C) Suppression of TF, TNF, GBP6 and IRF8 mRNA induction by NAPc2 (200 nM) and NAP5 (200 nM) after 1 hour of stimulation with immunoglobulin (10 μg/mL) isolated from patients with COVID-19 (n = 10), ∗P < .0001; 1-way analysis of variance (ANOVA).$

Inhibition of aPL prothrombotic and proinflammatory response by NAPc2. (A) Thrombus formation in mice treated with immunoglobulin (10 μg) fractions either alone or together with NAPc2; platelets are shown in red, and leukocytes are shown in green. Quantification of thrombus size in the vena cava inferior 3 hours after injection of immunoglobulin isolated from healthy controls (n = 4) or patients with COVID-19 (n = 6) and flow restriction. Mean ± standard deviation (SD), ∗∗∗∗P < .0001, t test following Shapiro-Wilk test for normal distribution. (B) MM1 cells were cultured in human serum or plasma and then stimulated for 1 hour with HL5B or HL7G (400 ng/mL each), rJGG9 or IgG (1 μg/mL each), LPS (10 ng/mL) or IgG from patients with COVID-19 (10 μg/mL). (C) Suppression of TF, TNF, GBP6 and IRF8 mRNA induction by NAPc2 (200 nM) and NAP5 (200 nM) after 1 hour of stimulation with immunoglobulin (10 μg/mL) isolated from patients with COVID-19 (n = 10), ∗P < .0001; 1-way analysis of variance (ANOVA).

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