Transcriptomic comparison of WHO_new, MD-WHO_est, MP-WHO_est, and healthy controls. (A) Principal component analysis of all included WHO_new, MD-WHO_est, MP-WHO_est, and healthy control samples. (B) Unsupervised clustering of 33 WHO_new and 193 MD- and MP-WHO_est cases. (C) Overlap between significantly differentially expressed genes in the WHO_new, MD-WHO_est, and MP-WHO_est cohorts compared against healthy controls. (D) Volcano plot depicting differential gene expression between the WHO_new and MD-WHO_est cohorts. The dotted line denotes the FDR cutoff at 0.05. (E) Normalized enrichment score of the 19 significantly enriched hallmark gene sets highlighted in dark blue. FDR, false discovery rate; logFC, log fold change; PC, principal component; NES, normalized enrichment score.