Production of LMP-TCs. In brief, donor peripheral blood mononuclear cells (PBMCs) are sorted into B cells, T cells, and monocytes. B cells are transfected with laboratory strain EBV and grown into the lymphoblastoid cell line (LCL). LCL and monocytes are transduced with a replication-incompetent adenoviral vector expressing LMP 1 and 2. T cells are cocultured with LMP1 and 2 expressing LCLs and monocytes in the presence of interleukin-2 (IL-2) to produce a LMP-specific T-cell product containing both CD4⁺ and CD8⁺ cells.