snRNA-seq reveals an interferon-responsive oligodendrocyte population in the cGVHD brain. (A) UMAP plots depicting the expression of various genes related to the identity of oligodendrocyte precursor cells (OPCs) and oligodendrocytes within the oligodendrocyte population. (B) UMAP plot depicting subclustering of OPCs & oligodendrocytes split by sample type. (C) Bar plot indicates the representation of each OPC/oligodendrocyte subcluster across the sample types. (D) Heat map depicting the average gene expression of the 44 genes identified as differentially expressed between population 0 and 2 in WT GVHD samples. (E) Volcano plot for WT GVHD oligodendrocytes in population 2 vs population 0. Plots shows -log₁₀ of the adjusted P value and average log₂ fold change (FC) for all genes, with highlighting of those that are significantly upregulated (avg_log2FC > 0.6 + P < .05, red) or downregulated (avg_log₂FC < 0.6 + P > .5, blue) in oligodendrocytes from population 2 vs populations 0. Gray dots indicate genes that are not differentially expressed.