Figure 5.
Poly(I:C) stimulates migration and type 1 IFN induction via MAVS. (A) Schematic overview of Poly(I:C) signaling pathways via TLR3 or MAVS. (B) Migration of peritoneal macrophages from C57BL/6J mice stimulated with Poly(I:C) (25 μg/mL) on fibrinogen-coated filters in the presence of TLR3/dsRNA complex inhibitor (27 μM) (C) Migration of peritoneal macrophages from C57BL/6J WT mice and Mavs–/– mice stimulated with Poly(I:C) (25 μg/ml) or CpG-B (5 μM) on fibrinogen-coated filters. (D) Gene induction of TNFα, TF, IFNα and IFNβ by Poly(I:C) (10 μg/mL) of macrophages plated on fibrinogen from F7flflLysMcre mice in comparison to littermate F7flfl controls. (E) Migration of peritoneal macrophages on fibrinogen-coated filters from F7flflLysMcre and F7flflCx3Cr1cre mice in comparison to littermate F7flfl controls stimulated with CpG-B (5 μM). (F) FXa generation with anti-TF 43D8, anti-TF 21E10, or control mouse IgG2a on MC38 cells (G) IFNα and IFNβ secreted into the cell supernatant by peritoneal macrophages from C57BL6J mice treated with anti-TF 43D8 IgG2a (50 μg/mL) or control mouse IgG2a (50 μg/mL). (H) Migration of peritoneal macrophages from C57BL/6J mice stimulated with Poly(I:C) (25 μg/mL) on fibrinogen-coated filters in the presence of anti-TF 43D8 IgG2a (50 μg/mL) or control mouse IgG2a (50 μg/mL). (I) Secreted TNFα, IFNα, and IFNβ in the cell supernatant of WT peritoneal macrophages plated on fibrinogen-coated plates and stimulated with Poly(I:C) (10 μg/mL) and treated with p38 inhibitor (SB 203580, 250 nM) or with ERK inhibitor (CAS 1049738-54-6 25 μM). (J) Migration of peritoneal macrophages stimulated with Poly(I:C) (25 μg/mL) on fibrinogen-coated filters with p38 inhibitor (SB 203580, 250 nM) or with ERK inhibitor (CAS 1049738-54-6 25 μM). Mean ± SD; the 1-way ANOVA with the Tukey multiple comparison test (B,I,J); the 2-way ANOVA with the Sidak multiple comparison test (C,D,E,G,H). ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001.

Poly(I:C) stimulates migration and type 1 IFN induction via MAVS. (A) Schematic overview of Poly(I:C) signaling pathways via TLR3 or MAVS. (B) Migration of peritoneal macrophages from C57BL/6J mice stimulated with Poly(I:C) (25 μg/mL) on fibrinogen-coated filters in the presence of TLR3/dsRNA complex inhibitor (27 μM) (C) Migration of peritoneal macrophages from C57BL/6J WT mice and Mavs–/– mice stimulated with Poly(I:C) (25 μg/ml) or CpG-B (5 μM) on fibrinogen-coated filters. (D) Gene induction of TNFα, TF, IFNα and IFNβ by Poly(I:C) (10 μg/mL) of macrophages plated on fibrinogen from F7flflLysMcre mice in comparison to littermate F7flfl controls. (E) Migration of peritoneal macrophages on fibrinogen-coated filters from F7flflLysMcre and F7flflCx3Cr1cre mice in comparison to littermate F7flfl controls stimulated with CpG-B (5 μM). (F) FXa generation with anti-TF 43D8, anti-TF 21E10, or control mouse IgG2a on MC38 cells (G) IFNα and IFNβ secreted into the cell supernatant by peritoneal macrophages from C57BL6J mice treated with anti-TF 43D8 IgG2a (50 μg/mL) or control mouse IgG2a (50 μg/mL). (H) Migration of peritoneal macrophages from C57BL/6J mice stimulated with Poly(I:C) (25 μg/mL) on fibrinogen-coated filters in the presence of anti-TF 43D8 IgG2a (50 μg/mL) or control mouse IgG2a (50 μg/mL). (I) Secreted TNFα, IFNα, and IFNβ in the cell supernatant of WT peritoneal macrophages plated on fibrinogen-coated plates and stimulated with Poly(I:C) (10 μg/mL) and treated with p38 inhibitor (SB 203580, 250 nM) or with ERK inhibitor (CAS 1049738-54-6 25 μM). (J) Migration of peritoneal macrophages stimulated with Poly(I:C) (25 μg/mL) on fibrinogen-coated filters with p38 inhibitor (SB 203580, 250 nM) or with ERK inhibitor (CAS 1049738-54-6 25 μM). Mean ± SD; the 1-way ANOVA with the Tukey multiple comparison test (B,I,J); the 2-way ANOVA with the Sidak multiple comparison test (C,D,E,G,H). ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal