TF-FVIIa-PAR2 signaling promotes migration on fibrinogen. (A) Migration of peritoneal macrophages and BM monocytes from C57BL/6N mice stimulated with Poly(I:C) (25 μg/mL) on fibrinogen-coated filters in the presence of inhibitory αMβ2 (M1/70, 25 μg/mL) antibody or control IgG. (B) Migration of macrophages and monocytes from Itgb1^flflLysM^cre and littermate Itgb1^flfl WT control mice stimulated with Poly(I:C) on fibronectin-coated filters. (C-E) Migration of macrophages and monocytes from F2rl1^R38E, F7^flflLysMcre, F7^flflCX3CR1cre, F10^flflLysMcre, and WT control mice stimulated with Poly(I:C). (F) Inhibition of migration on fibrinogen with PAR2 antagonist AZ3451 (1 μM). (G) Migration of macrophages and monocytes from C57BL/6N mice with inhibitors anti-TF21E10 (25 μg/ml) or 5L15 (50 nM). (H) Fold induction of Poly(I:C) induced migration with and without 5 nM mouse FVII (mFVII) added to macrophages from F7^flflLysMcre mice. Mean ± SD; the 1-way ANOVA with the Tukey multiple comparison test (A,F,G); the 2-way ANOVA with the Sidak multiple comparison test (B,C,D,E,H). ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001.