FVII is required for Poly(I:C)-induced monocyte/macrophage recruitment to lung exudates. (A) FVII plasma levels in F7flflLysMcre vs in littermate F7flfl mice determined by clotting assay. (B) F7 mRNA and FVII-secreted protein in isolated peritoneal macrophages from F7flflLysMcre and F7flflCx3Cr1cre mice in comparison with littermate F7flfl controls. (C) Schematic overview of the standard experimental model of lung inflammation induced by intranasal Poly(I:C) application. (D) Schematic model of PAR2 activation by the TF-FVIIa-FXa complex involving the endothelial protein C receptor (EPCR). (E) Cell counts in BAL in unchallenged and Poly(I:C)-treated F10flflLysMcre and littermate F10flfl mice. (F) Schematic overview of PAR2 activation by the TF-FVIIa complex involving integrins. (G) Cell counts in BAL in unchallenged and Poly(I:C)-treated F7flflLysMcre and F7flfl littermate control mice. (H) Cell counts in BAL in F7flflCx3Cr1cre and F7flfl mice. (I) Cell counts in BAL of mice 24 hours after receiving 1 or 2 doses of Poly(I:C). Mean ± standard deviation (SD); the 2-way ANOVA with the Sidak multiple comparison test (E,G,I) or the unpaired Student t test (H). ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001. Mo, monocytes; MՓ, macrophages.
