LDL-R expression is low on human HSCs in the BM of humanized mice. (A) LDL-R and CD46 expressions on human CD34⁺ cells in steady-state BM of humanized NBSGW mice. Mean fluorescence intensity (MFI) corrected by the fluorescence-minus-1 (FMO) control is displayed; n = 5; error bars indicate SD. (B) Representative histogram of LDL-R, CD46, and FMO control (gray) signals detected from flow cytometry of the samples in panel A. (C) Schematic of in vitro culture, stimulation, and flow-cytometry analysis using steady-state human donor BM and G-CSF–mobilized human CD34⁺ cells. (D) LDL-R signals of steady-state human donor BM samples corrected by FMO signals. Data from 2 independent donors are displayed. (E) LDL-R signals of G-CSF–mobilized human CD34⁺ and CD34⁺CD90⁺ cells corrected by FMO signals.