VSV-G-LV transduces mature blood cells and progenitors in vivo. (A) Schematic of generating a humanized NBSGW mouse model and injecting a lentivirus encoding GFP in the BM. (B) Percent human CD45⁺ cells in the murine BM. (C-E) Percent human CD34⁺, CD34⁺CD38^low, and CD34⁺CD90⁺ cells in the BM of noninjected and injected limbs. Shaded in gray are the range of values from control (mock) animals. (F) Percent GFP⁺ cells of human CD45⁺ cells. (B-F) Treated (injected/noninjected), n = 4; and control, n = 3. (G) Percent human CD34⁺, CD34⁺CD38^low, and CD34⁺CD90⁺ cells of GFP⁺ cells (n = 4). (H) Representative plots showing the gating strategy used to characterize the GFP⁺ population.