Assessment of peripheral blood for immune reconstitution. Flow cytometry immune monitoring was performed on peripheral blood samples collected from patients on Ven/Aza maintenance and matched controls at 1 (n = 51, including 26 study patients and 25 controls), 2 (n = 41, including 24 study patients and 17 controls), 3 (n = 47, including 24 study patients and 23 controls), 6 (n = 38, including 18 study patients and 20 controls), and 12 (n = 34, including 13 study patients and 21 controls) months after transplant. (A) T-cell populations were measured at serial time points after transplant to evaluate for impaired immune cell expansion. Three major T-cell populations, regulatory CD4⁺ T (CD4Treg), conventional CD4⁺ T (CD4Tcon), and CD8⁺ T cells, were defined as CD3⁺CD4⁺CD8⁻CD25⁺CD127⁻ cells, CD3⁺CD4⁺CD8⁻CD25^−/lowCD127^+/− cells, and CD3⁺CD4⁻CD8⁺, respectively. (B) Natural killer (NK) cells were defined as CD3⁻CD56⁺ lymphocytes. NK T cells were defined as CD3⁺CD56⁺ lymphocytes. B cells were defined as CD45⁺CD19⁺ lymphocytes. Details of gating strategy are shown in supplemental Figure 5. Data are median (thick black line), and box plots indicate the interquartile range.