Evolution of changes in RBC O2-handling kinetics during kidney perfusion. (A) RBC O2-unloading time constant measured at various points (x-axis) during perfusion for the 30 cases (along y-axis). (B) RBC O2-unloading capacity measured at various points (x-axis) during perfusion for the 30 cases (along y-axis). (C) Analysis of stored blood at the end of perfusion. Time constant frequency distribution (green line shows reference blood mean). (D) Analysis of stored blood at the end of perfusion. Capacity frequency distribution (green line shows reference blood mean). (E) Effect of kidney perfusion on RBC oxygen-unloading time constant (significant decrease; paired t test) and capacity (no significant change; paired t test). (F) Initial oxygen-unloading rate calculated from the time constant and capacity. “End” denotes measurement towards end of perfusion; “pre” denotes packed RBCs prior to perfusion.
