Lenalidomide and dexamethasone therapy induces multimerization of vWF. (A) Representative electrophoresis (above) and densitometric analysis (below) of vWF multimers in patients undergoing treatment including lenalidomide (UPN #6) and off-treatment (UPN #7). The OD pattern was analyzed using ImageJ 1.53t. Peaks 1 through 5 represent the small multimers, and peaks 6 through 10 represent the intermediate multimers. The large and ultra-large multimers have >10 peaks. (B) Comparison of the proportion of intermediate and large multimers to total vWF multimers between patients during treatment including lenalidomide (n = 3) and off-treatment (n = 4). Each area of vWF multimer peaks in the OD analysis was measured by ImageJ 1.53t. Statistical analysis was performed by Wilcoxon 2-sample exact test. Len, lenalidomide; UPN, unique patient number.