Figure 4.
Lenalidomide and dexamethasone synergistically increase intracellular THBS-1 level. (A) Relative mRNA expression levels of THBS1 in THP1 cells. Cells were treated with DMSO, 1 μM dexamethasone with or without 10 μM lenalidomide for 24 hours. Data are from 3 independent experiments and expressed as means ± SEM. P values were determined by 2-tailed Student t test. (B) Immunoblot analysis for THBS-1 in THP1 cells treated for 24 hours with each drug. (C) THBS-1 concentration in the culture medium of PBMNCs. PBMNCs from healthy donor were cultured in RPMI-1640 for 24 hours with or without 1 μM dexamethasone and 10 μM lenalidomide, and THBS-1 concentration of medium was evaluated by ELISA assay. (D) Serial changes in THBS-1 protein level of PBMNCs from case 1 to 5 listed in supplemental Table 1. Cont, control; Dex, dexamethasone; IB, immunoblotting; Len, lenalidomide; Pom, pomalidomide; UPN, unique patient number.

Lenalidomide and dexamethasone synergistically increase intracellular THBS-1 level. (A) Relative mRNA expression levels of THBS1 in THP1 cells. Cells were treated with DMSO, 1 μM dexamethasone with or without 10 μM lenalidomide for 24 hours. Data are from 3 independent experiments and expressed as means ± SEM. P values were determined by 2-tailed Student t test. (B) Immunoblot analysis for THBS-1 in THP1 cells treated for 24 hours with each drug. (C) THBS-1 concentration in the culture medium of PBMNCs. PBMNCs from healthy donor were cultured in RPMI-1640 for 24 hours with or without 1 μM dexamethasone and 10 μM lenalidomide, and THBS-1 concentration of medium was evaluated by ELISA assay. (D) Serial changes in THBS-1 protein level of PBMNCs from case 1 to 5 listed in supplemental Table 1. Cont, control; Dex, dexamethasone; IB, immunoblotting; Len, lenalidomide; Pom, pomalidomide; UPN, unique patient number.

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