Altered levels of plasma proteins relevant for cell signaling, neutrophil activation, and angiogenesis in individuals with ADAN. Analysis of 249 plasma proteins using proximity ligation assay (Olink) and ELISA. (A-E) Twenty-eight proteins significantly differed in individuals with ADAN from those in HDs, of which 22 were downregulated and 6 were upregulated in the group with ADAN. (A) Volcano plot showcasing the fold change in altered proteins between those with ADAN and HDs. (B) Significance heat map of the 28 proteins. (C-D) Raw NPX values or concentrations (TNF) for selected proteins. (E) Selected identified terms in Gene Ontology analysis (biological process) and the contributing proteins. Nonparametric testing (Wilcoxon rank-sum test). HDs (n = 6-20) and individuals with ADAN (n = 5-7). Olink Proteomics arbitrary unit, in Log2 scale. Samples for plasma Olink and other inflammation/autoimmune analyses were obtained at apparently infection-free intervals, as assessed by CRP and the treating physician. FDR, false discovery rate; NPX, Normalized Protein eXpression.