Increased fractions of CD14⁺CD16⁺ monocytes in individuals with ADAN. Cells from individuals with ADAN (ADAN) (n = 7) and age-matched healthy donors (HDs) (n = 11) were analyzed by flow cytometry. (A) Frequency of monocytes, DC, and NK cells among the PBMC. (B) The frequencies of myeloid DC (mDC) and plasmacytoid DC (pDC) among DCs. (C-D) Monocyte subsets defined according to their expression of CD16 and SLAN or in (D) according to the traditional gating based on CD14 and CD16 expression. Statistical significance of the differences between mean values was calculated using the Mann-Whitney test, and P-values < 0.05 are indicated.