Phenotypic characterization of CD19 CAR T cells. (A) Flow cytometry analysis depicting CD4⁺ and CD8⁺ populations of CD3⁺CD34⁺ cells transduced after IL-7 culture or CD3 activation. (B) CD8:CD4 ratio of CD3⁺CD34⁺ cells transduced after IL-7 culture or CD3 activation. The graph depicts each data point from 10 donors with lines connecting data points from an individual donor. Statistics were calculated by 2-tailed Wilcoxon rank sum test. ∗P = .0355. (C) Flow cytometry analysis depicting the T_SCM (CD62L⁺CD45RA⁺CCR7⁺CD95⁺), T_CM (CD62L⁺CD45RA⁻), T_EM (CD62L⁻CD45RA⁻), and T_EFF (CD62L⁻CD45RA⁺) populations of starting material and CD3⁺CD34⁺ cells transduced after IL-7 culture or CD3 activation. (D) Percentage of T_SCM and T_EFF populations in CD3⁺CD34⁺ cells transduced after IL-7 culture or CD3 activation. The graphs depict each data point from 5 donors with lines connecting data points from an individual donor. Statistics were calculated by 2-tailed Wilcoxon rank sum test. T_SCM ∗P = .0159; T_EFF ∗P = .0465. Graphs in panel A are representative of 10 experiments using cells from 10 different donors, whereas graphs in panel C are representative of 5 experiments using cells from 5 different donors.