Manipulation of Nfkbiz expression by ASO alters HSC lineage priming toward myeloid lineage. (A) Schematic representation of ASO (shown in red) for Reg1/Reg3-targeting SL structures in the mouse Nfkbiz 3’ UTR. (B) Experimental design for ASO-transduced HSCs. HSCs were electroporated with control oligo or Nfkbiz-targeting ASO. Cells were used for subsequent analyses in (C-H). (C-D) qRT-PCR analysis of Nfkbiz, Reg1, Reg3 and HSC/lineage-related genes in cultured HSCs at day 5 after electroporation (n = 5 each). (E-F) Representative flow cytometry plots and frequency of FcγR expression in CD48^–CD150⁺ LSK from cultured HSCs stimulated with IL-1β or TNF for 24 hours (n = 3 each). (G-H) Representative flow cytometry plots and frequency of FcγR⁺CD11b⁺ cells from cultured HSCs stimulated with IL-1β or TNF for 24 hours (n = 3 each). (I) Model of the Reg1/Reg3-Nfkbiz axis in the control of HSC lineage priming. Data are representative of 3 independent experiments (C-H). Data are presented as mean ± SD (C-D,F,H) Statistical significance was calculated by unpaired 2-tailed Student t test (C-D,F,H).