Effect of the inhibition of specific kinases on membrane proteins tyrosine phosphorylation and morphology of OV-treated RBCs. RBCs from RCCs (n = 3) were analyzed on day 6. Mean values ± SD are displayed. (A) WB analysis against pY-containing membrane proteins of RBCs treated with 2 mM OV and 10 μM KI. (A, left) Images of the blots; (A, right) total relative pY signal (ie, sum of pY-positive bands normalized by total protein loading [ponceau red]). (B) Morphology analysis by DHM. AUC was obtained by integration of the SD-OPD over time. P values, Dunnett multiple comparison test: ns P > .05; ∗P ≤ .05; ∗∗P ≤ .01. (C) Single-cell analysis with CellProfiler and CellProfiler Analyst. RBCs were classified as “Stomatocytes,” “Discocytes,” “Echinocytes,” or “Spherocytes.” (C, top) Evolution over time per cell type; (C, bottom) integration over time. P values, Šidák multiple comparison test: ns P >.05; ∗P ≤ .05; ∗∗P ≤ .01; ∗∗∗P ≤ .001, ∗∗∗∗P ≤ .0001.