Timelapse analysis by DHM of the morphological changes triggered by 2 mM OV treatment. RBCs from RCCs (n = 3) stored for 6, 19, and 40 days were imaged at 20× original magnification, mean values ± SD are displayed. P values, Tukey multiple comparison test: ns P > .05. (A) Population analysis: (A, left) evolution of SD-OPD signal for 3 RCCs over a 15-hour timelapse analysis; (A, right) AUC obtained by integration of the SD-OPD over time. (B) Phase images illustrating the changes of RBC phenotype. (C) Single-cell analysis with CellProfiler and CellProfiler Analyst. RBCs were classified as “Stomatocytes,” “Discocytes,” “Echinocytes,” or “Spherocytes.”