Membrane proteins tyrosine phosphorylation, microvesiculation, and hemolysis in OV-treated RBCs. RCCs (n = 6) stored for 1, 21, and 42 days were analyzed, mean values ± SD are displayed on the graphs. P values, Tukey multiple comparison test: ns P > .05; ∗P ≤ .05; ∗∗P ≤ .01; ∗∗∗P ≤ .001. (A) WB analysis against pY-containing membrane proteins of RBCs treated 2 mM OV for 1 or 4 hours. (A, left) Representative image of WB experiment (RCC 2), note that the molecular weight marker bands (M) were superimposed on the chemiluminescence pictures; (A, right) Total relative pY signal (ie, the sum of pY-positive bands normalized by total protein loading [ponceau red]). (B, left) Concentration of microvesicles released; and (B, right) level of hemolysis after 1 or 4 hours of treatment.