Phosphoproteomics of OV-treated RBC membrane proteins. RBCs from RCCs at day 2 of storage (n = 6) were analyzed. (A) Phosphoproteome-depth: number of identified phosphoproteins, phosphopeptides, phosphosites, and class 1 phosphosites. The number of phosphosites (class 1 or quantified class 1) are presented in different colors of blue. (B) Comparison of phosphosites after 1 hour of OV treatment vs without any treatment. Volcano plot showing t test P values (log10) vs phosphosite fold changes (log2). Blue points correspond to class 1 phosphosites (multiplicity 1) differentially quantified, and unchanged phosphosites are in gray. Significant pY are highlighted in red and nonsignificant in light red. The black curves correspond to a FDR < 0.05 and a S0 = 1. (C) Protein-protein interactions of upregulated and downregulated (the 2 on the bottom left) phosphoproteins.