Cystine depletion results in lipid peroxidation. (A) Lipid peroxidation was quantified by C11-BODIPY staining upon 16 or 24 hours of cystine depletion or after 16 or 24 hours of cystine depletion but in the presence of 0.5 μM DPI; both normalized to the control condition in which cystine was present. The mean fluorescence intensity (MFI) fold change of oxidized C11-BODIPY is shown. (B) Representative histogram plots of data shown in panel A. (C) Lipid peroxidation was quantified by C11-BODIPY staining upon 16 or 24 hours of cystine depletion but in the presence of 1.0 mM GSH; both normalized to the control condition in which cystine was present. The MFI fold change of oxidized C11-BODIPY is shown. (D) Lipid peroxidation was quantified by C11-BODIPY staining upon 16 or 24 hours of cystine depletion but in the presence of 0.5 mM HCY; both normalized to the control condition in which cystine was present. The MFI fold change of oxidized C11-BODIPY is shown.