Study design and clinical outcome. (A) Schematic representation of the clinical trial design, treatments, and biopsy schedule and overview of assays performed on the obtained specimens. (B) Waterfall plot depicting best response in SPD of either all target lesions (blue bars) or uninjected, distant target lesions only (green bars). All 3 patients with progressive disease progressed at a single site of disease but experienced tumor reduction elsewhere. (C) Spider plot depicting change in SPD of all target lesions over time from treatment initiation. CT, computed tomography; FNA, fine needle aspirate; RT, radiotherapy; SPD, sum of product of diameters; IWG, international working group.