FUS^high HSCs exhibit global chromatin reorganization. (A) Heatmaps showing the normalized Hi-C interaction frequencies of the whole genome (1 Mb bin) of FUS^low and FUS^high HSCs (CD34^–LSK) from young (2-4 months old) Fus-gfp mice (2 biological replicates for each group) (left) and differential interactions between them (right). (B) The decaying curve shows the genome-wide contact probability for interactions within individual chromosomes by genomic distance. The dotted line represents 6 Mb. (C) The boxplot showing the ratio of proximal and distal contacts (PvD) for young, aged, FUS^low, and FUS^high HSCs. (D) Saddle plots of Hi-C data binned at 100 kb resolution showing the compartmental interactions for FUS^low HSCs (top left) and FUS^high HSCs (top right), and the differential interactions between them (bottom right). The differentially interacting compartments are marked by the dashed lines. (E) These curves show the insulation score around TAD boundaries in young, aged, FUS^low, and FUS^high HSCs (±1 Mb). (F) These boxplots show the contact probability of intra-TAD and inter-TAD interactions for FUS^low and FUS^high HSCs. (G) Aggregation analysis for TADs showing the interactions for FUS^low (top left) and FUS^high HSCs (top right) and differential interactions between them (bottom right), wherein blue denotes loss of interactions and red denotes gain of interactions in FUS^high HSCs. (H) Example of TAD fusion and insulation score distribution for chromosome 17 (84-88 Mb) in FUS^low and FUS^high HSCs.