American Society of Hematology

Figure 3.

$FUShigh HSCs resemble aged HSCs. (A) Experimental design of a competitive transplantation assay for FUSlow and FUShigh HSCs. 35 freshly isolated FUShigh and FUSlow HSCs (CD34–CD150+LSK) from young (2-4 months old) Fus-gfp mice were transplanted into lethally irradiated recipients (CD45.2) together with 3.5 × 105 competitors (CD45.1). The chimera in peripheral blood was evaluated every 4 weeks until week 16. (B) These line plots depict the percentage of donor-derived cells (overall, B cell, T cell, myeloid cell) in the peripheral blood of the recipients at the indicated time points. N = 7 recipients per group, data are shown as mean ± SEM. (C) The histogram depicts the lineage distribution of donor-derived peripheral blood cells at the 16th week after transplantation. N = 7 recipients per group, data are shown as mean ± SEM. (D-E) These figures show the GSEA of aging-related genes and myeloid differentiation–related genes (D) and HSC fingerprint genes between FUSlow and FUShigh HSCs (CD34–CD150+LSK). NES, normalized enrichment score. |NES| > 0.3 and P < .05 represent significant difference. The genes are listed in supplemental Table 2. (F) Experimental design of competitive transplantation assay for young, aged, aged FUSlow, and aged FUShigh HSCs (CD34–LSK). A total of 100 freshly isolated HSCs from 3 young (2-month-old) Fus-gfp mice and 100 FUShigh HSCs, 100 FUSlow HSCs, 100 unfractionated HSCs from 2 aged (18-month-old) Fus-gfp mice were transplanted into lethally irradiated recipients (CD45.2) together with 3.5 × 105 competitors (CD45.1). The chimera in peripheral blood was evaluated every 4 weeks until week 12. The experiment was repeated 2 times. (G) These line plots depict the percentage of donor-derived cells (overall, B cell, T cell, myeloid cell) in peripheral blood of the recipients at indicated time points. N = 4-7 recipients per group, data are shown as mean ± SEM. (H) The histogram depicts the lineage distribution of donor-derived peripheral blood cells at week 12 after transplantation. N = 4-7 recipients per group, data are shown as mean ± SEM.$

FUS^high HSCs resemble aged HSCs. (A) Experimental design of a competitive transplantation assay for FUS^low and FUS^high HSCs. 35 freshly isolated FUS^high and FUS^low HSCs (CD34^–CD150⁺LSK) from young (2-4 months old) Fus-gfp mice were transplanted into lethally irradiated recipients (CD45.2) together with 3.5 × 10⁵ competitors (CD45.1). The chimera in peripheral blood was evaluated every 4 weeks until week 16. (B) These line plots depict the percentage of donor-derived cells (overall, B cell, T cell, myeloid cell) in the peripheral blood of the recipients at the indicated time points. N = 7 recipients per group, data are shown as mean ± SEM. (C) The histogram depicts the lineage distribution of donor-derived peripheral blood cells at the 16th week after transplantation. N = 7 recipients per group, data are shown as mean ± SEM. (D-E) These figures show the GSEA of aging-related genes and myeloid differentiation–related genes (D) and HSC fingerprint genes between FUS^low and FUS^high HSCs (CD34^–CD150⁺LSK). NES, normalized enrichment score. |NES| > 0.3 and P < .05 represent significant difference. The genes are listed in supplemental Table 2. (F) Experimental design of competitive transplantation assay for young, aged, aged FUS^low, and aged FUS^high HSCs (CD34^–LSK). A total of 100 freshly isolated HSCs from 3 young (2-month-old) Fus-gfp mice and 100 FUS^high HSCs, 100 FUS^low HSCs, 100 unfractionated HSCs from 2 aged (18-month-old) Fus-gfp mice were transplanted into lethally irradiated recipients (CD45.2) together with 3.5 × 10⁵ competitors (CD45.1). The chimera in peripheral blood was evaluated every 4 weeks until week 12. The experiment was repeated 2 times. (G) These line plots depict the percentage of donor-derived cells (overall, B cell, T cell, myeloid cell) in peripheral blood of the recipients at indicated time points. N = 4-7 recipients per group, data are shown as mean ± SEM. (H) The histogram depicts the lineage distribution of donor-derived peripheral blood cells at week 12 after transplantation. N = 4-7 recipients per group, data are shown as mean ± SEM.

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