Fga^EK/EK mice have preserved hemostatic potential. (A) Time to cessation of bleeding (sustained >30 seconds) of Fga^WT/WT, Fga^WT/EK, Fga^EK/EK, Fga^+/−, and Fga^−/− mice after 3-mm excision of the distal portion of the tail. Horizontal bar indicates the mean with data analyzed using the Kaplan-Meier log-rank test. (B) Time to cessation of bleeding of Fga^WT/WT, Fga^WT/EK, Fga^EK/EK, and Fga^−/− mice after laser-induced saphenous vein injury (n = 18-23 per genotype). Horizontal bar indicates the mean, with data analyzed using one-way ANOVA. (C) Representative images of platelet plug (green), fibrin(ogen) (red), and merged panels 5 minutes after laser-induced saphenous vein injury. (D) Representative 3-dimensional reconstruction of injury sites depicting the side view after injury. Each grid box = 50 μm × 50 μm. Quantification of (E) fibrin(ogen) and (F) platelet accumulation at the site of injury. Data are expressed as the mean ± SEM, and analyzed using one-way ANOVA. ∗P < .05; ∗∗P < .01;  ∗∗∗P < .001. s, second.