Fga^EK/EK and Fga^WT/EK mice are protected from venous thrombosis. (A) Incidence of thrombus formation and (B) thrombus weights from Fga^WT/WT, Fga^WT/EK, and Fga^EK/EK mice 24 hours after IVC ligation (stasis model). (C) Circulating fibrinogen levels of before and after IVC ligation measured by enzyme-linked immunosorbent assay. (D) Spearman correlation analysis of circulating fibrinogen level after ligation, and thrombus mass. Data are presented as the mean ± SEM and analyzed using Kruskal-Wallis test. (E) Representative images of 5-μm-thick sections of thrombi stained against fibrin(ogen) (green), CD41 (red), and DAPI (4′,6-diamidino-2-phenylindole) (blue). Note that Fga^EK/EK thrombi contained minimal platelets and nucleated cells as well as morphologically distinct aggregates of fibrinogen compared with Fga^WT/WT and Fga^WT/EK thrombi that display web-like fibrin matrixes. ∗P < .05; ∗∗∗P < .001.