Gata2 germline variation attenuates combinatorial HSPC mobilization. (A) Mice were given 8 doses of 125 mg/kg rhG-CSF over 5 days followed by vehicle, 5 mg/kg plerixafor, 1 mg/kg rhIL-8, or 1 mg/kg BIO5192 and harvested after 1 hour. (B) CFU from 0.1 mL of peripheral blood. (C) Peripheral blood neutrophil quantification. (D) Splenic LT-HSC (LSK CD150⁺ CD48^–) quantification. N = 3 to 10 per condition, 7 experiments. ∗P < .05, ∗∗P < .01, ∗∗∗P < .001; 2-tailed unpaired Student t test with Benjamini-Hochberg correction. (E) Repopulating activity in peripheral blood 16 weeks after competitive transplant of 50 μL peripheral blood with 10⁶ BM competitor cells. N = 5 to 6 per condition from 2 experiments. ∗P < .05, ∗∗∗P < .001; 1-way ANOVA followed by Tukey multiple comparisons test. (F-G) Monoallelic Ets motif variation attenuates mobilization. WT, monoallelic (Ets^+/–), homozygous biallelic (Ets^–/–), and CH variants were tested. CFU from 0.1 mL of peripheral blood. N = 3 to 6 per condition, 6 experiments. ∗P < .05, ∗∗P < .01, ∗∗∗P < .001; 1-way ANOVA followed by Tukey multiple comparisons test. PBS, phosphate-buffered saline.