Gata2 germline variation blocks HSPC expansion after acute G-CSF treatment. (A) Configuration of Gata2 variant alleles in WT and CH. (B) Mice were treated with 1 dose of 125 mg/kg rhG-CSF or vehicle and harvested after 4 or 16 hours. (C) Representative flow cytometric plots for BM HSPC quantification. The numbers represent the percentage of the parent cell population. N = 6 to 9 per condition, 4 experiments (D) BM HSPC quantification. HSC: Lin⁻ Sca1⁺ Kit⁺ (LSK) CD48^– CD150⁺; MPP: LSK CD48^– CD150^–; LSK CD48⁺; Lin^– Sca1^– c-Kit⁺. ∗P < .05, ∗∗P < .01, ∗∗∗P < .001; 1-way ANOVA followed by Tukey multiple comparisons test. FSC, forward scatter; PBS, phosphate-buffered saline.