Cdc73 promotes DNA repair and OXPHOS gene expression through its gene body functions. (A-C) Box and whisker plots of CDC73 (A), ETS1 (B), and Notch/RBPJ (C) tag counts in human THP-6 cells at gene bodies (A) and promoters (B-C) of all genes, OXPHOS genes, and DNA repair genes shared by the GSEA enrichment cores in Cdc73^f/f 969 and 970 T-ALL cells (Figure 3I; supplemental Figure 4G; supplemental Table 1). (D-E). Violin plots showing H2BK120ub1 ChIP-seq Log2FC in 969 cells (D) and 970 cells (E) for all genes, DNA repair genes, and OXPHOS genes in the GSEA enrichment cores of 969 and 970 cells (supplemental Table 1). OHT was added for 30 hours to delete Cdc73. (F-H) Metagene plots of H2BK120ub1 signals in EtOH-treated (blue) and OHT-treated (red) 969 cells at all genes (F), DNA repair genes (G), and OXPHOS genes (H) in core enrichment genes of GSEA analyses (supplemental Table 1). (I-L) Display tracks of indicated ChIP-seq and ATAC-seq datasets at important DNA repair genes (I-J) and OXPHOS genes (K-L) in mouse 969 cells (top) or human THP-6 cells (bottom) showing representative tracks and FDR values upon OHT addition (Cdc73 deletion) of H2BK120ub1 signals between transcriptional start site (TSS) and transcriptional termination site (TTS). ∗∗∗FDR < 0.001; ∗∗∗∗FDR < 0.0001. ATAC-seq (GSM2461649). Ets1 ChIP-seq (GSM2461515).