Cdc73 is important for OXPHOS. (A-B) Representative flow cytometric histogram (A) and mean florescence intensity scatterplot (N = 3) (B) showing mitochondrial membrane potentials measured by tetramethylrhodamine methyl ester (TMRM) assay on live 4′,6-diamidino-2-phenylindole (DAPI)-negative Rosa26CreER^T2Cdc73^f/f T-ALL cells (970) treated with OHT for 30 hours to delete Cdc73. FCCP was added as a positive control. (C-D) Representative flow cytometric histogram (C) and mean fluorescence intensity scatterplot (N = 3) (D) showing mitochondrial reactive oxygen species (ROS) production measured by the CellROX assay on live DAPI-negative 970 cells treated with OHT for 30 hours to delete Cdc73. TBHP was added as a positive control. (E-M) Seahorse XFe96 instrument measurements of real-time oxygen consumption rate (OCR) normalized to live cell number and protein concentration under basal conditions or in response to the indicated mitochondrial inhibitors (E,H,K) and scatterplots of basal (F,I,L) and adenosine triphosphate (ATP) production (G,J,M) respiration phases of 970 cells treated with OHT for 30 hours to delete Cdc73 (E-G), control Rosa26CreER^T2 cells treated with OHT for 30 hours (H-J), and CUTLL1 cells at 4 days after transduction with 2 independent shCDC73 (K-M).