Bidirectional repopulation assays of Runx^R188Q/+ hematopoietic cells. (A-B) Analysis of contribution of WT donor cells (CD45.1) to peripheral blood (A) and BM (B) after 24 weeks, into WT (orange) or Runx^R188Q/+ (green) recipient mice (CD45.2), using bidirectional noncompetitive repopulation assays. (C-D) Time-course analysis of donor cell contribution (test/competitor: WT/WT in blue, Runx1^R188Q/+ /WT in red) to peripheral blood in wt (C) and Runx1^R188Q/+ (D) recipient mice, in bidirectional competitive repopulation assay (BCRA). ∗P < .05, ∗∗P < .005; ns, not significant.