GSK3$β$abrogation in hematopoietic cells does not significantly alter hematopoietic cell maturation. (A) Example of polymerase chain reactionperformed on mouse DNA to confirm the genotype. (B) Protein levels of GSK3$β$⁠, GSK3$α$⁠, and $β$-actin as assessed by western blot from mouse total bone marrow cell lysate (n = 4 per genotype, in which WT is Cre⁻ GSK3$βf/f$ and KO is Cre⁺ GSK3$βf/f$⁠). (C) GSK3β abrogation does not lead to any significant changes in white or red blood cell counts by CBC analysis ± standard error mean (SEM) (n = 8 per condition). (D) Morphologic analysis of mouse bone marrow aspirate does not reveal any differences between GSK3$β$ WT and KO mice. Bone marrow aspirate was stained with Wright-Giemsa stain, and images were captured at original magnification ×50 (n = 4 per condition). (E) No differences in bone marrow morphology were observed between GSK3$β$ WT and KO mice. Representative bone marrow sections were stained with hematoxylin and eosin (H&E), and images were captured at original magnification ×4 (n = 4 per condition). (F) No differences in spleen morphology were observed between GSK3$β$ WT and KO mice. Representative spleen sections were stained with H&E, and images were captured at original magnification ×4 (n = 4 per condition). (G) No differences in spleen mass were observed between GSK3$β$ WT and KO mice (n = 5 per condition). (H) No differences in the frequency of HPCs between GSK3$β$ WT and KO mice were observed in peripheral blood samples. Average frequency of hematopoietic cell populations from mouse bone marrow as measured by flow cytometry (n = 4 per condition). CMP, common myeloid progenitor; f/f, floxed/floxed; f/w, floxed/wild-type; GMP, granulocyte-monocyte progenitors; Hb, hemoglobin; LK, c-kit low cells; LMPP, lymphoid-primed multipotent progenitors; LSK, Lineage⁻ Sca-1⁺ c-Kit⁺cells; LT-HSC, long-term-HSC; Ly, Lymphocyte; MO, Monocyte; NE, Neutrophil; RBC, red blood cell; ST-HSC, short-term-HSC; WBC, white blood cell; w/w, wild-type/wild-type.