Human fetal-lung explants undergo EHT to produce HSPCs. (A) Image of a human fetal-lung explant culture showing a robust population of floating hematopoietic cells against a background of adherent cells. (B) Cytospin of human fetal lung suspension cells. (C) Flow cytometric assessment of differentiated erythrocyte and megakaryocyte populations. (D) Images of representative colonies from a colony-forming unit assay. (E) Assessment of pre-HSC and EHT markers on CD34⁺/CD45⁺–defined progenitors vs CD34^–/CD45⁺–differentiated hematopoietic cells.