Fetal lung EHT is functionally reliant on canonical developmental pathways. (A) Violin plots of expression of TGFβ/BMP pathways genes. (B) Representative flow cytometry plots demonstrating the change in CD45⁺/VECAD⁺ progenitors after treatment with the Notch inhibitor compound E, the TGFβ inhibitor SB431542, and recombinant TGFβ2. (C) Counts of CD45⁺/VECAD⁺ progenitors after treatment. (D) Average MFI of VECAD expression after treatment. (E) Representative histograms of VECAD expression after treatment. Error bars represent standard deviation. ∗∗P < .01 compared with DMSO control. Each n represents a biological replicate with a minimum of 3 technical replicates per experiment.