Murine fetal-lung explants exhibit the dynamics of EHT. (A) Flow cytometric assessment of VECAD and CD45 expression on day 5 fetal-lung explants. (B) CD45 and VECAD expression across days 4 to 6. (C) Assessment of SLAM marker–defined HSPC populations stratified by VECAD expression across multiple days in culture. (D-E) Assessment of pre-HSC and EHT markers on VECAD⁺/CD45⁺–defined progenitors and separated by adherent vs suspension cell populations. Each n represents a biological replicate with a minimum of 3 technical replicates per experiment. Error bars represent standard error. ∗P < .05, ∗∗P < .01.