Renal injury markers are elevated in allo-HCT mice. (A-E) BALB/c mice received 7 Gy of TBI on day −1, and received transplantation with 0.5 × 10⁶ CD90.2⁺ splenic T cells together with 5 × 10⁶ BM cells from either syngeneic (syn) BALB/c or allogeneic (allo) MHC-mismatched B6 donors. (A-C) n = 6 to 10 mice per group, pooled from 2 experiments. (D-E) n = 3 to 5 mice per group; representative data from 3 experiments are shown. (F-J) B6D2F1 mice received 11 Gy of TBI on day −1 and received transplantation with 3.5 × 10⁶ CD90.2⁺ splenic T cells together with 5 × 10⁶ BM cells from either syngeneic B6D2F1 or allogeneic parent-to-F1 B6 donors. (F-H) n = 6 to 10 mice per group, pooled from 2 experiments. (I) n = 4 to 5 mice per group, (J) n = 3 to 5 mice per group; representative data from 2 experiments are shown. (K-O) C3H.sw animals received 10.5 Gy of TBI on day −1 and received transplantation with 1 × 10⁶ CD90.2⁺ splenic T cells together with 5 × 10⁶ TCD-BM cells from either syn C3H.sw or MHC-matched multiple minor antigen–mismatched B6 donors. (K-M) n = 6 to 10 mice per group, pooled from 2 experiments. (N-O) n = 3 to 5 mice per group; representative data from 2 experiments are shown. Survival (A,F,K), clinical GVHD score (B,G,L), body weight change (C,H,M), and urinalysis (E,J,O) are shown. Urinary NAG levels of recipient mice on day 14 after BMT was significantly higher in the allogeneic group in the MHC-mismatched (D) and parent-to-F1 (I) models than in other models. (N) In the MHC-matched multiple minor antigen–mismatched model, urinary NAG levels tended to be higher in the allo group than in the syn group. Results summarize at least 2 independent experiments. Data represent the mean ± standard error of the mean (SEM). Student t test was used for statistical analysis; ∗P < .05; ∗∗P < .01; ∗∗∗P < .001. UOB, urinary occult blood; UP, urinary protein.