Figure 3.
HAPLN1 matrikine activates STAT1 in MM cells. (A) The graph depicts Mining Algorithm for GenetIc Controllers–identified enrichment scores of transcription factors and cofactors in response to PTR1 treatment in RPMI8226 cells. (B) Reads per kilobase million values of indicated genes from the RNA-seq results are plotted. (C) STAT-dependent luciferase reporter activities on RPMI8226 cells with the indicated stimuli are shown. The graph depicts the mean ± SEM of the quantification of 3 independent replicates. (D) Representative STAT1 and STAT3 supershift analysis of RPMI8226 cells incubated with MBP or MBP-PTR1 (100 nM; 6 hours) or IL-6 (50 ng/mL; 15 minutes). (E-F) Representative western blot analysis on RPMI8226 cells treated with the indicated dose of MBP or MBP-PTR1 for 6 hours (E) or 100 nM MBP-PTR1 for the indicated time (F). ∗P < .05.

HAPLN1 matrikine activates STAT1 in MM cells. (A) The graph depicts Mining Algorithm for GenetIc Controllers–identified enrichment scores of transcription factors and cofactors in response to PTR1 treatment in RPMI8226 cells. (B) Reads per kilobase million values of indicated genes from the RNA-seq results are plotted. (C) STAT-dependent luciferase reporter activities on RPMI8226 cells with the indicated stimuli are shown. The graph depicts the mean ± SEM of the quantification of 3 independent replicates. (D) Representative STAT1 and STAT3 supershift analysis of RPMI8226 cells incubated with MBP or MBP-PTR1 (100 nM; 6 hours) or IL-6 (50 ng/mL; 15 minutes). (E-F) Representative western blot analysis on RPMI8226 cells treated with the indicated dose of MBP or MBP-PTR1 for 6 hours (E) or 100 nM MBP-PTR1 for the indicated time (F). ∗P < .05.

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