Spatial transcriptional correlation analysis between immune, epithelial, and endothelial compartments in aGVHD of colon. Canonical correlation analyses (CCAs) were performed to visualize genes that correlate together across the different compartments of (A) epithelium and immune AOIs; (B) immune and vascular endothelium AOIs; and (C) epithelium and vascular endothelium AOIs. The axes (termed canonical components or canonical covariates) were constructed to maximize the correlation between gene counts in immune and epithelial AOIs in the same ROI. On this CCA plot, each dot represents a gene. Each gene’s coordinate is determined by its correlation with the first (x-axis) and second (y-axis) canonical components. The correlation between 2 genes is positive if the angle between them is sharp (acute) and negative if the angle is obtuse.