In the absence of Zbtb11, FL-committed progenitor numbers fail to increase. (A) Schematic description of the analysis. Flow cytometry analysis dot plots show representative incidence at E17.5: (B/B′) LMPP (LSKCD34⁺Flt3⁺); (D/D′) CMP (Lin⁻CD127⁻Sca-1⁻c-Kit⁺CD34⁺CD16/32⁻), GMP (Lin⁻CD127⁻Sca-1⁻c-Kit⁺CD34⁺CD16/32⁺), and MEP (Lin⁻CD127⁻Sca-1⁻c-Kit⁺CD34⁻CD16/32⁻); and (H/H′) CLP (Lin⁻CD127⁺Sca-1⁺cKit⁺Flt3⁺). Scatter plots showing the absolute quantification of total content at E14.5, E16.5, and E17.5: (C) LMPP, (E) CMP, (F) GMP, (G) MEP, and (I) CLP. For panels C and I: E14.5 WT, n = 22 and KO n = 7; E16.5 WT, n = 16 and KO n = 4; E17.5 WT, n = 8 and KO n = 2. For panels E-G: E14.5 WT, n = 22 and KO, n = 7; E16.5 WT, n = 16 and KO, n = 4; E17.5 WT, n = 16 and KO, n = 5. WT/controls (green); KO, Zbtb11^hKO (red); data ± standard error of the mean. Two-way analysis of variance with Tukey (black) or Šídák (blue) multiple comparisons: ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001. CLP, common lymphoid progenitor; GMP, granulocyte–macrophage progenitor; LMPP, lymphoid-primed multipotent progenitor; MEP, myeloerythroid progenitor.