Inhibition of DHODH leads to cell cycle arrest and apoptosis in T lymphoblasts. (A) Treatment of T-ALL cell lines with BRQ, showing dose-dependent decreases in cell viability, as measured by CellTiter-Glo. Shown are the mean ± standard deviation of 3 replicates. (B) T-ALL cell lines were treated with 1 μM BRQ. Graph showing a time-dependent increase in annexin V staining measured by flow cytometry. Shown are data from a representative experiment, which was repeated 3 times. (C) Treatment of T-ALL cell lines in vitro with 1 μM BRQ for 72 hours resulted in an S-phase arrest. Shown are data from a representative experiment, which was repeated 3 times.