IFN-α treatment differentially impacts ischemic stroke outcomes in young and old mice. (A) Mice were treated for 3 days with either vehicle or IFN-α. Twenty-four hours after the induction of transient middle cerebral artery occlusion in young (age, 4-6 months) and old (age >24 months) mice, survival was monitored (B), and neurological (a higher score is worse) (C) and motor function (a lower score is worse) (D) were assessed. (E) Brain tissue was stained with TTC. Infarcted tissue (white) is outlined with a black dotted line. (F) Ischemic stroke brain damage was quantified by planimetric analysis of TTC stained brain slides. All data are represented as mean ± standard deviation. TTC, triphenyltetrazolium chloride.