FMT from young mice reduced inflammation and promoted lymphoid differentiation in aged LT-HSCs. (A) Volcano plot for DEGs in LT-HSCs. (B) Violin plots showing the expression of inflammation-related genes, stemness-related genes, and lymphoid-specific genes. (C) Representative gene ontology (GO) analysis results enriched in upregulated DEGs of LT-HSCs in the FMT-YA group. (D) Representative gene set enrichment analysis enrichment plots of GO terms in LT-HSCs. (E) Circle plot showing differential interaction strength in the cell-to-cell communications between FMT-YA and FMT-AA. Blue-colored edges represent decreased signaling in the FMT-YA data set and red-colored edges represent increased signaling in the FMT-YA data set. (F) Enrichment analysis results of differential ligand-receptor pairs targeting LT-HSCs between FMT-YA and FMT-AA. (G) Chord diagram showing downregulated signaling ligand-receptor pairs targeting LT-HSCs in FMT-YA. (H-K) Circle plot showing the source of inflammatory cytokines. Edge weights are proportional to the interaction strength. (L) Bar graphs showing the BM serum concentrations of IL-1β, TNF-α, and CXCL1. Graphs show mean ± SEM (n = 6 per group), with statistical significance determined by Student t test (n = 6 per group). ∗P < .05, ∗∗P < .01.