A transcriptome-wide landscape of hematopoiesis after FMT by scRNA-seq. (A) Flowchart for cell collection and data analysis in scRNA-seq. BM cells were harvested from FMT-AA mice and FMT-YA mice. Whole BM cells, immature hematopoietic cells (Lin⁻), and hematopoietic progenitor cells (Lin⁻ cKit⁺) were collected by flow cytometric sorting and admixed at a 1:1:4 ratio and then subjected to 10X Genomics Chromium capture platform. (B) Uniform manifold approximation and projection (UMAP) plot showing the clustering results of hematopoietic cells. (C) Pseudotime trajectory analysis of hematopoiesis from HSCs to mature hematopoietic cells. (D) Bubble plot showing the expression of key marker genes in different cell clusters. (E) Bar graph showing the cell ratios of hematopoietic cells in FMT-YA and FMT-AA mice, based on scRNA-seq. (F-G) Pie charts showing the relative abundance of HSPC subpopulations and hematopoietic lineage differentiation in FMT-YA and FMT-AA mice. DC, dendritic cell; CMP, common myeloid progenitor; GMP, granulocyte/monocyte progenitor; MEP, megakaryocyte/erythroid progenitor; Mo, monocyte.