Tregs did not inhibit the upregulation of gene sets involved in the GVT effect. (A) Transcript expression of effector molecules (Ifng, Il2, Tnf) in CD4 Tcons. (B) Enrichment plots displaying enrichment scores for the genes involved in leukocyte cytotoxicity (GO_LEUKOCYTE_MEDIATED_CYTOTOXICITY) from CD4 Tcons recovered at day 8 after HCT in the presence or absence of Tregs. (C) Transcript expression of effector molecules (Ifng, Gzmb, Prf1) in CD8 Tcons. (D) Enrichment plots displaying enrichment scores for the genes involved in leukocyte cytotoxicity (GO_LEUKOCYTE_MEDIATED_CYTOTOXICITY) from CD8 Tcons recovered at day 8 after HCT in the presence or absence of Tregs. Gene signatures were obtained from MSigDB.