Paired transcriptomic analysis of Tcons and Tregs identified mechanisms of GVHD suppression. (A) Transcript expression of genes encoding for molecules involved in classical Treg mechanisms of suppression. (B) Enrichment plots displaying enrichment scores for the genes involved in Tgfb (HALLMARK_TGF_BETA_SIGNALING), Il10 (REACTOME_INTERLEUKIN_10_SIGNALING), Il35 (GSE24210_CTRL_VS_IL35_TREATED_TCONV_CD4_TCELL_DN), and Il2 (HALLMARK_IL2_STAT5_SIGNALING) in CD4 (left) and CD8 (right) Tcons recovered at day 8 after HCT in the presence or absence of Tregs. Gene signatures were obtained from the Molecular Signatures Database (MSigDB).