Tregs modulated CD4 and, to a lesser extent, CD8 Tcon transcriptome during GVHD. (A) Principal component analysis of transcriptome based on the top 1000 differentially expressed genes across all CD4 (left) and CD8 (right) Tcon samples. (B) Volcano plots showing significance and log2 fold change of transcripts from Tregs treated CD4 (left) and CD8 (right) Tcons compared with those from untreated Tcons. Vertical dashed lines on volcano plots indicate a log2 fold change of 1.5; horizontal dashed line indicates an adjusted P value = .05.